What is the optimal pH and temperature ranges when using Mix&Go? The pH of Mix&Go solution is 2.8 and Mix&Go-activated beads are stable in this environment. Our data indicates that protein-coupled Mix&Go beads are compatible with commonly used buffers/solutions (with the notable exception of PBS for storage) including: * 100 mM MES pH 5.2 and pH 6.0 * 100 mM Tris pH 8 * 50 mM acetate-buffered saline pH 5 * 150 mM normal saline * Mix&Go interaction withstands 2% SDS at 100oC for 10 min PBS* *Note: PBS is not a problem for Mix&Go beads during assay conditions such as assay buffer
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