MixnGo Sigma Product, page-16

Hi cara

I think it is 12 months but best to check with the company

Details at end of article below

RB



Mix&Go™ Biosensor
AI-PLSC010-04.00
Page 1 of 2
Mix&Go™ Biosensor has been developed for binding antibodies and other proteins to polystyrene, silica, ceramic
and other planar surfaces commonly used in biosensors.
Considering the variety of materials used as biosensor
surfaces, optimisation is recommended when testing different materials. Where stringent uniformity of proteins are
required at the surface interface, Mix&Go Biosensor has many advantages over existing methods such as
EDC/NHS chemistry or hydrophobic interactions.
Materials Supplied Additional Materials Required
Cat. No. Component
A-PLSC010 Mix&Go™ Biosensor Surface to coat
Protein of interest
Pipettes
Coating Buffer (25 mM MES pH 6)
Wash Buffer (PBS 0.05% Tween20®)
Blocking Buffer (5% BSA in Coating Buffer)
Deionised water (ddH2O)
Compatibility
Buffers It is recommended that a range of antibody coating buffers are tested with Mix&Go Biosensor to
achieve optimal results. Avoid using buffers or additives with strong chelation potential (e.g. PBS,
EDTA). A recommended buffer for use with Mix&Go is 25mM MES pH 6.
Mix&Go reagent is not suitable for use as a coating buffer or antibody diluent.
Temperature Do not freeze or expose to temperatures exceeding 60ºC.
Room temperature is defined as 20ºC - 25ºC.
Protein Concentration Protein coupling concentration is best optimised as this can vary depending on the protein used.
Automation Many automated apparatuses may contain surfaces that Mix&Go reagent can bind to. The solution
should be tested using manual procedures before introduction into an automated system. Take
caution of accidental exposure of Mix&Go reagent to bottles, glassware and other surfaces.
Surfaces Mix&Go Biosensor has been used to activate polystyrenes, certain cyclic olefin copolymers (COC),
microarray slides (glass), silicon oxides, titanium oxides, ceramics and gold surfaces (excluding gold
colloids in suspension). Mix&Go Biosensor is not compatible with certain materials including
polypropylene (PP), and certain thermoplastics.
Safety
Standard safety precautions exercised when handling laboratory reagents should be adhered to. Refer to the MSDS for safety
precautions.
Warnings/Hazards
Product is not guaranteed DNase, RNase or endotoxin free. The user must determine the suitability of the product for specific
uses.
Other Products
For more information on Anteo products please visit www.anteotech.com.
Specifications
Ordering Information A-PLSC010-5 (5 mL)
A-PLSC010-10 (10 mL)
A-PLSC010-50 (50 mL)
Storage Store at 20°C - 25°C.
Remaining materials should be retained in the supplied container and sealed for future use.
pH 3.15 - 3.35
Stability Surfaces activated and coupled by the user should be assessed for individual use and storage
stability conditions, as this can vary depending on the surface, protein and conditions used.
Activated surfaces should be stored in a dry chamber or pouch using desiccant or similar materials
to minimise humidity.
Mix&Go Biosensor should not appear to change colour or form precipitates.
Applications Mix&Go Biosensor is for use on
polystyrene, silica, ceramic and other planar surfaces commonly
used in biosensors.
Regulatory For laboratory use only.
Instructions For Use Effective Date: 11/09/2015
Review Date: 11/09/2016
Mix&Go™ Biosensor
AI-PLSC010-04.00
Page 2 of 2
Example Procedure Time
Activation

1. Allow reagents to come to room temperature
2. Coat the required surface with Mix&Go Biosensor (Cat. No. A-PLSC010) ensuring that the necessary

surface areas are adequately covered

1. Cover and incubate at room temperature 30 minutes
2. Wash the surface with ddH2O
3. Dry the surface

Coupling
i At this stage do not treat the surface with chemicals or buffers with strong chelation potential, as Mix&Go will bind to
these agents.

1. Coat the required surface with protein in coating buffer ensuring that the necessary surface areas

are adequately covered

1. Cover and incubate at room temperature 60 minutes

Washing

1. Wash the surface with wash buffer

Blocking

1. Coat the required surface with blocker in coating buffer ensuring that the necessary surface areas

are adequately covered

1. Cover and incubate at room temperature 30 minutes

Washing

1. Wash the surface with wash buffer

Storage

1. Dry the surface and store in dry chamber or pouch with desiccant

i Store coupled surface at 2ºC - 8ºC.
Observations
Mix&Go Biosensor allows antibodies and other proteins to bind faster and with more functionality. In some cases, surfaces
may be activated with Mix&Go in as little as 5 minutes and proteins can bind to Mix&Go activated surfaces in as little as 10
minutes at room temperature. Some optimisation of incubation times and protein concentration is recommended to utilise the
full advantage that Mix&Go has to offer.
Contact
Website: www.anteotech.com
Technical Support: [email protected]
Bulk Pricing & Sales Enquiries: [email protected]
Anteo Technologies Pty Ltd Office: +61 7 3219 0085
Unit 4, 26 Brandl Street Fax: +61 7 3219 0553
Eight Mile Plains QLD 4113
Australia
All Anteo Products are sold as general purpose reagents for general laboratory and research uses only. Anteo Products are not intended for diagnostic and/or therapeutic purposes and no
Anteo Product may be administered to humans. Anteo does not make any representation or warranty that Anteo Products comply with all laws and regulations that may be applicable to
Customer’s use of any Anteo Product.
Anteo warrants that Anteo Products will conform to the specifications set forth on the applicable Anteo Product Certificate of Analysis (“Anteo’s Limited Warranty”). The Certificate of
Analysis may be obtained by contacting Anteo. Anteo’s Limited Warranty is wholly conditioned on the proper use of Anteo Products in the applications for which they are intended, and Anteo
makes no warranty (express, implied or statutory) for Anteo Products that are modified; subjected to accident, misuse, neglect, unauthorized repair or improper tampering, testing or storage;
and/or used or handled contrary to Anteo’s instructions as set forth on the Anteo Product label and/or in this insert.
Unless otherwise expressly provided in this document or in these Terms and Conditions of Sale, Anteo disclaims all warranties, conditions and guarantees, whether written, express, implied,
statutory or otherwise, including but not limited to, the implied warranties or guarantees of merchantability and fitness for particular purpose.
Tween® is a registered trademark of ICI Americas.
Mix&Go™ is a registered Australian trademark of Anteo Technologies Pty Ltd.