Just skim read through this..
Visualization of brain microvasculature and blood flow in vivo: Feasibility study using confocal laser endomicroscopy
Seems for blood flow there was an issue of 'pressure artifacts' which seems to cause problems for the researchers with this type of medical procedure.
Way above my knowledge base !And they summarised with .
Overall, CLE presents with a learning curve that must be overcome to develop an adequate probe-handling technique and to navigate smoothly to desired locations for image acquisition without significant artifacts.
Their final comment though was..
Research and clinical scenarios may benefit from CLE as a novel intraoperative real-time in vivo microscopic-resolution fluorescence imaging modality.
doi: 10.1111/micc.12678
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