Sorry, mate. Busy day and wanted to reply properly. The...

Sorry, mate. Busy day and wanted to reply properly.

The bioluminescence represents tumor burden in NSG mice engrafted with MOLM-13-luc AML cells, with the color intensity indicating the extent of tumor growth. The color scale on the right side of the images ranges from purple to red, where purple and blue denote lower levels of bioluminescence, corresponding to smaller tumor burden or less active tumor cells. Green and yellow indicate progressively higher levels of bioluminescence, reflecting greater tumor burden and higher metabolic activity of the tumor cells. Red, as you might have expected is the highest level of tumor burden and metabolic activity of the tumor cells. As tumor cells proliferate, they produce more luciferase, leading to increased bioluminescence upon injection of D-luciferin. Therefore, areas displaying green to red colors signify a higher tumor burden, with red being the most intense and indicative of significant tumor growth. In this model, the images clearly show that the combination of DAC (Decitabine) and ZAN (Bisantrene) treatment results in a reduced bioluminescence signal, suggesting a lower tumor burden compared to the control and single-treatment groups.

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