Read preso at this link that Twin put up earlier. Outlines the diff between first ver and the optimised assay.......sounds to me like Mn'G is in there now, but I can't be certain of that.
Limited shelf life....
Modification of the plate coating protocol....
http://future-diagnostics.com/wp-content/uploads/2016/04/Poster-Free-Vitamin-D.pdf
Ann: DIAsource Receives Grant to Develop Free Vitamin D Assay, page-44
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