hey Factor, The article can be found here. How Antibody Surface...

hey Factor,

The article can be found here.
How Antibody Surface Coverage on Nanoparticles Determines the Activity and Kinetics of Antigen Capturing for Biosensing
http://pubs.acs.org/doi/abs/10.1021/ac501536z

Received: April 8, 2014
Accepted: July 21, 2014

Here is part of the opening paragraph of the intro that is highly relevant to us:

“In all cases, the antigen-capturing activity of the antibodies is crucial and this strongly depends on the immobilization strategy. The process to anchor the antibody molecules to the particle surface is a delicate balance between, on the one hand, the need to reliably attach the antibodies to the nanoparticle surface, and on the other hand, the need to keep the antibodies in a biologically active molecular conformation with a high accessibility for capturing antigens from solution. A stable link is needed between antibody and particle, while minimally changing the antibody molecule and without reducing its interaction with the solution.”

Also in the conclusion, relevant: "As a next step, the developed supernatant assay methodology can be applied to quantitatively characterize the capturing functionality of other surface biofunctionalizations"

All IMO, dyor.