Ann: Investor Update, page-67

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P$Ychic

518 Posts.

20/12/14

16:13

Post #: 14491427
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Originally posted by pallister ↑

Benzene dioxygenase and toluene dioxygenase from Pseudomonas putida have similar catalytic properties, structures, and gene organizations, but they differ in substrate specificity, with toluene dioxygenase having higher activity toward alkylbenzenes. The catalytic iron-sulfur proteins of these enzymes consist of two dissimilar subunits, alpha and beta; the alpha subunit contains a [2Fe-2S] cluster involved in electron transfer, the catalytic nonheme iron center, and is also responsible for substrate specificity. The amino acid sequences of the alpha subunits of benzene and toluene dioxygenases differ at only 33 of 450 amino acids. Chimeric proteins and mutants of the benzene dioxygenase alpha subunit were constructed to determine which of these residues were primarily responsible for the change in specificity. The protein containing toluene dioxygenase C-terminal region residues 281 to 363 showed greater substrate preference for alkyl benzenes. In addition, we identified four amino acid substitutions in this region, I301V, T305S, I307L, and L309V, that particularly enhanced the preference for ethylbenzene. The positions of these amino acids in the alpha subunit structure were modeled by comparison with the crystal structure of naphthalene dioxygenase. They were not in the substrate-binding pocket but were adjacent to residues that lined the channel through which substrates were predicted to enter the active site. However, the quadruple mutant also showed a high uncoupled rate of electron transfer without product formation. Finally, the modified proteins showed altered patterns of products formed from toluene and ethylbenzene, including monohydroxylated side chains. We propose that these properties can be explained by a more facile diffusion of the substrate in and out of the substrate cavity.

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Now you're adding plagiarism to your list of accomplishments. At least provide the link to the above in your post... ps fyi... it was originally posted in 13 July 2004. Whatever it means..

http://aem.asm.org/content/71/3/1570.short

PSY are hardly going to employ Dr Jason Chaffey as Director of Commercialisation
and Manufacturing to bring the product to market if he hasn't already got a product to work with. As someone has previously stated he's not there to make the coffee!!

Now go away and play with your mining stocks... you little scientific genius you. You're going on the "ignore troll" list where you belong.

PSY Price at posting: 26.5¢ Sentiment: Buy Disclosure: Held
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