Ann: NUZ-001 Promotes Survival in TDP-43 Challenged NSC-34 Cells, page-4

Hi HC, I'm a bit confused now. This paragraph says that survival has been enhanced but NUZ-001 shows no effect on autophagy markers.

"Preliminary data suggest that NUZ-001 had a small but significant effect on TDP-43-mediated cell death in the mouse
MSC-34 motor neuron cell line. Despite enhancing survival, acute treatment with NUZ-001 showed no effect on
autophagy markers, suggesting that an additional pharmacological process may be at play. These data also suggest
that the neuroprotective effects of NUZ-001 may require repeated exposure providing further insight into its overall
biological effects. Future studies will evaluate the effects of NUZ-001 under specific treatment conditions to further
elucidate the biological mechanism of action."


What does this actually mean? I thought we had convincingly proved that NUZ-001 was promoting autophagy. Is it saying that NUZ-001 had no effect on the MSC-34 motor neuron cell line and therefore must be working its magic in another way?


thanks