Nice find Slura. Results significantly increased by up to 45 times using Anteo Coupling compared to traditional methods. RSV is huge, and this study appears to be able to measure infections over a long period using Anteo Coupling. We have always believed the Anteo tech is in a league of its own, which this study proves.
”Improved G protein antibody detection through Anteo Coupling. All RSV antigens were coupled using either EDC/s-NHS or Anteo (see “Materials and methods” section for details) in a variety of concentrations to determine optimal performance. While most antigens were unaffected or showed minimal changes in response to these different methods/concentrations in mean fluorescence intensity (MFI), there was a significant improvement in G protein performance when Anteo coupling was used (Fig. 1). Compared to classical EDC-sNHS coupling, Anteo coupling resulted in significant increases in MFI values (all p < 0.001), with subtype A G proteins increasing by 7.3–45.6×-fold and subtype B G proteins increasing by 1.5–5.4×-fold “
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