Ann: Publication comparing Cymerus MSCs to other MSCs, page-46

Induced pluripotent stem cells (iPSCs) haverecently boomed enthusiasm in stem cell therapy, whereas high potentialtumorigenesis of iPSCs has become the biggest obstacle for clinic applicationand the tumorigenic genes in iPSCs have not been well documented. In thisinvestigation, using tools of bioinformatics, we analyzed the all availabledatasets regarded to iPSCs from 11 differentiated cell lines and revealed 593iPSC consensus genes. Notably, of the 593 genes, 209 were expressed in humantumor cell lines and cancer tissues, and some of them were expressed in theiPSC-differentiated hepatocytes; remarkably, 5 oncogenes were overexpressed inthe iPSCs and an oncogene RAB25 in the iPSC-differentiatedcells, suggesting that these iPSC consensus genes are implicated with the riskof tumorigenesis and cancers. This investigation provides useful informationfor designing new strategies and methods to curtail the expression of oncogenicgenes in iPSCs and produce safe iPSC derivatives for stem cell therapy.