Ann: Quarterly Activities Report and Appendix 4C, page-48

Hi JB1975,

Will a HEK293 cell line - that's human embryonic kidney if I recall correctly produce the same exosomes as say are of interest in mesenchymal stromal cell therapies?

Can I ask why you're comparing HEK293 with MSC for the production of exosomes? I believe it's been established that different cell lines will produce exosomes with different characteristics. And even exosomes derived from the same cell line can be (in fact, will be) heterogenous.

Exopharm establishing a cGMP Master Cell Bank for HEK293 is promising. From a Feb 2022 paper -

HEK293-derived exosomes show a remarkable ability to carry and deliver chemotherapeutic drugs, such as doxorubicin, in such a manner that the drug causes minimal toxicity to non-targeted cells.

And

Ohno et al. demonstrated the tumor-targeting property of exosomes isolated from HEK 293 cells by decorating them with the GE11 peptide or epidermal growth factor (EGF) on their surface, and then used them to deliver let-7a miRNA to EGFR-expressing breast cancer xenograft tissues leading to an enhanced therapeutic effect.

https://www.sciencedirect.com/science/article/pii/S1044579X22000475