Cell Therapy News/Articles, page-17381

Seems back in 2019 investigating Extracellular Vesicle dosing vs MSCs in mice they cooked up their own with this recipe using fat from hernia operations (human hernias, not mice...but some mice do pump iron and don't lift properly). Might whip some up tonight for dinner.

"...Briefly, healthy human adult subcutaneous adipose tissue was obtained during open ventral hernia repairs of healthy patients defined as no history of malignancy or IBD. The subcutaneous adipose pearls were micro-dissected, washed in phosphate-buffered saline (PBS) to remove erythrocytes, minced, and digested with 0.25% type I collagenase (Gibco Life Technologies, Waltman, MA) at 37 °C for 60 min under constant shaking. Cells were pelleted, supernatant was removed, and cells were resuspended and cultured in xeno-free, serum-free MSC NutriStem® XF Medium (Biological Industries USA, Cromwell, CT, cat# 05-200-1A-KT) under standard cell culture conditions of 37 °C/5% CO2. The cultured MSCs were grown to 85–90% confluency in T75 tissue culture flasks before passaging. MSCs were finally harvested at passage 3 to 4."