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On ADOs'FAQ page:Can Mix&Go coupled beads withstand harsh...

  1. oslo
    5,687 Posts.
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    31/05/14
    07:32
    Post #: 13441223
    On ADOs'FAQ page:

    Can Mix&Go coupled beads withstand harsh conditions, such as used in protein purification or immunoprecipitation?

    Yes. Mix&Go coupled beads withstand all of the below conditions:

    Boiling beads for 10 minutes in 2% SDS-PAGE sample buffer (SDS, DTT, Glycerol, Coomassie Blue dye)

    Treating beads for 48 hours with 0.5M EDTA then boiling them in SDS-PAGE buffer

    Storing Mix&Go-activated beads in 200mM EDTA for 1 year

    Extremes of pH ~ 2.5 (Glycine or Citrate buffer elution) to pH ~ 12 (0.5M NaOH washing)

    ADO Price at posting: 23.0¢ Sentiment: LT Buy Disclosure: Held
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    lightbulb Created with Sketch.  Great analysis
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