Something to look forward to with Smith and Nephew for the...

Something to look forward to with Smith and Nephew for the remainder of this year and next year as well?

www.dtic.mil/descriptivesum/Y2012/DARPA/0601101E_1_PB_2012.pdf
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Evaluation, Defense-Wide
BA 1: Basic Research
R-1 ITEM NOMENCLATURE
PE 0601101E: DEFENSE RESEARCH
SCIENCES PROJECT
BLS-01: BIO/INFO/MICRO SCIENCES

Title: Biological Adaptation, Assembly and Manufacturing
B. Accomplishments/Planned Programs ($ in Millions)
FY 2010 $7.738 FY 2011 $9.482 FY 2012 $8.386
Description: The Biological Adaptation, Assembly and Manufacturing program will examine the structure, function, and informational basis underlying biological system adaptation, and the factors employed by the organism to assemble and manufacture complex biological subsystems.

The unique stability afforded biological systems in their ability to adapt to wide extremes of physical and endurance (e.g., heat, cold, and sleeplessness) parameters will be examined and exploited in order to engineer stability into biological systems required for the military (such as blood, bioengineered tissues or other therapeutics).

In addition, the fault tolerance present in biological systems will be exploited in order to assemble and manufacture complex physical and multi-functional systems, both biological and abiotic (such as tissue constructs designed for reconstructive surgery).

These systems include novel load-bearing bio-interactive materials and composites for repair of severe hard tissue trauma, including complex bone fractures. A key new antibody technology will develop the ideal antibody master molecule for use in unattended sensors that maintains high temperature stability and controllable affinity for threat agents.

Using the Freytag triangle structure,the interplay of narratives or stories may reveal how they tap into an array of mechanisms implicated in memory, reasoning,and strategy behavior. Applications to Defense systems include the development of chemical and biological sensors, tools for strategic military decision-makers involved in public relations and information operations, and improved warfighter battlefield survivability.

FY 2010 Accomplishments:
- Developed novel resorbable wet adhesives with the mechanical properties of natural bone, for inclusion into fracture putty formulation.
- Demonstrated fracture putty in small animal model of bone fracture.
- Initiated large animal studies of fracture putty for bone fracture repair.
- Identified fundamental mechanisms for controlling antibody stability and affinity.
- Initiated efforts to modify antibody affinity and temperature stability of the MS2 scFv antibody.
- Determined the baseline binding parameters of the anti-MS2 scFv and established the methodology for evaluating
improvements in antibody performance.

FY 2011 Plans:
- Demonstrate fracture putty in large animal model of bone fracture, with independent validation.
- Initiate expanded large animal studies of fracture putty in preparation for human clinical trials.
- Demonstrate the ability to produce an antibody with thermal stability from room temperature up to 60 degrees Celsius.
- Combine identified antibody stability and affinity capabilities into a single "Master Antibody Molecule" that exhibits two target metrics against a single biological threat agent and deliver a minimum of two grams for testing by a government laboratory.
- Incorporate the identified "Master Antibody Molecule" into an existing biosensor platform and demonstrate advanced capability in terms of robustness and potential for multiplexing.
- Initiate investigations into the relationship between dopaminergic-driven learning systems, hormones/neurotransmitters such as oxytocin, emotion-cognition interactions, and narrative structures.

FY 2012 Plans:
- Further investigate use of fracture putty in fixation and healing of large animal injury.
- Revise design of fracture putty compounds as appropriate for safety in human clinical trials.
- Explore and refine foundational assumptions on the utility of the Freytag structure ("setup-climax-resolution") for narrative
analysis, including determining relationships between decomposed stories and neuropsychological mechanisms, and
understanding relationships between narratives and behavior.
- Develop decomposition frameworks and initial cluster of neurobiological mechanisms to better understand their relationship.
- Develop tools to link analytic frameworks, neural mechanisms, and environmental variables to a particular story.