..akin to an in vitro (lab assumption) but when taken to in vivo ( the planet) can not be proven . In vitro to in vivo extrapolation
Main article: In vitro to in vivo extrapolation
Results obtained from in vitro experiments cannot usually be transposed, as is, to predict the reaction of an entire organism in vivo. Building a consistent and reliable extrapolation procedure from in vitro results to in vivo is therefore extremely important. Solutions include:
Increasing the complexity of in vitro systems to reproduce tissues and interactions between them (as in “human on chip” systems)[14]
Using mathematical modeling to numerically simulate the behavior of the complex system, where the in vitro data provide model parameter values[15]
These two approaches are not incompatible; better in vitro systems provide better data to mathematical models. However, increasingly sophisticated in vitro experiments collect increasingly numerous, complex, and challenging data to integrate. Mathematical models, such as systems biology models, are much needed
