Convo is far from over. Only getting started.Just wondering,...

Convo is far from over. Only getting started.

Just wondering, speaking of timelines, and you being the expert up to about 2015 McFly, any chance you could tell me which of your treatments was the world's first stem cell treatment on offer by (2004 + 8 years + 3 years) 2015:

"Itescu says he is not sure which of his products will pass regulatory scrutiny but the likelihood of bringing a treatment to market after a Phase 3 trial is about 50 per cent. He says given the company is planning several of these trials over the next year, Mesoblast could be offering the world's first stem cell treatment within three years."
https://www.aim.com.au/blog/silviu-itescus-hard-cell
There are a few more timelines that are worthwhile discussing as well.

Pott
Kettle
Black

In a 2017 Biocentury interview (I know, a few years in the future based on your research, but the maths is timeless) SI made the following statements and I can't seem to get my head around it:
https://www.biocentury.com/bc-innovations/product-rd/2017-07-20/cynata-preps-take-first-ips-cell-derived-mscs-clinic
"In standard protocols, MSCs are harvested from human bone marrow and adipose tissue, plated in culture, and then expanded from colony. Roughly 20,000 MSCs are recovered from a donor and amplified to billions of cells for trials and commercial prottduction."

Later on SI states (and I will come back to that a bit later), "I would much rather take a G0 cell and take it through 20 population doublings, than start with an iPS cell that has divided many times".

He makes a mention that a large clinical trial would require 2-3 donors, but of how many patients exactly does a large trial consist?

Anyways, based on the figures provided:

20,000 x 20 doublings = 20,971,520,000 cells

A treatment of Temcell (as stated before) requires on average between 16-24 bags with 72,000,000 cells per bag = 1,440,000,000 cells

= 20,971,520,000 / 1,440,000,000

= about 15 treatments per donor

x 3 donors = 45 patients (= large clinical trial?)

There are other research papers (e.g. Wagner et al), which showed that a super donor could possible result in a number of cells that would be equivalent to 500 treatments, that is however after 25 population doublings (that's when the senescence occured and and proliferation ground to halt). And if I work my way back from 25 doublings equalling 500 treatments, again, I come up with about 15 treatments after 20 doublings.
What is your definition of a large trial?

Also, congratulations on your latest partnership deal:



Cows in on one side, burgers out to the left, and the Fetal Bovine Serum out on the right. All ready now for commercialisation. All natural "stuff."

Your language reminds me of a great movie, can't remember the title though:



"Dr Bauer always said Ryoncil was like a box of chocolates. You never know what potency level you're gonna get."

Your ability to discover all these details and rephrase it, dumb it down for us Cynata holders is not lost on me. You manage to see so much more than us, even to the extent that you know better what the author meant or was meant to say than what he actually said/wrote. That's talent. I hope MSB is reading your posts and aware of your creativity. You could take over the ASX announcements in the future and report on all these collegial relationships you have built over the years.

Nah, this convo is far from over.