With everyone focused on the wait for results from BBI+POC1 is it possible that another large market for M+G has escaped notice?
A Google search on COC/COP shows that it is perhaps the new standard for high quality medical use
Replaces glass, non breakable, non-leaching, no tungsten, ETC
And in this article from Anteo M+G works wonders
RB
Simple protein immobilisation onto surfaces by multi-point avidity metal chelation
Yang L, Ling T, Vukovic P, Jennins D, Wen Ooi H, de las Heras R, Baumgartner T, Chung E, Ohse BT, Gao Y, Cooper S, Wong A, Munian C, Huang CY, Maeji, NJ
Anteo Diagnostics Ltd, Brisbane, Australia; www.anteodx.com
Surface attachment of biomolecules onto solid supports
utilised in biosensors is increasingly challenging, due in
part to the broadening range of surfaces used (e.g.
plastics, metals, glass and ceramics) and their
continued miniaturisation.
The use of conventional approaches such as passive
adsorption and covalent chemistries can result in poor
protein stability and poor control of loading density.
Together, these can adversely damage the immobilised
protein’s structure and function limiting the sensitivity of
the biosensor.
We have developed an alternative approach that
utilises a multi-point avidity metal chelation based
surface chemistry, named Mix&Go™ (Fig. 1). Mix&Go is
comprised of cationic metal polymers (<5,000 D) that
bind surfaces with electron donating potential, resulting
in the formation of thin film coatings (approx. 1 nm
thickness) that are very stable and robust. The
polymeric metal ions of Mix&Go chelate and bind by
multi-avidity to both the synthetic surface and to
biomolecules, thereby acting as a ”molecular glue".
Figure 1. Mix&Go molecular glue is comprised of polymeric
metal ions that chelate to available electron donating groups on
synthetic surfaces and proteins.
We demonstrate the utility of using Mix&Go to couple
proteins onto several surfaces whilst;
1. controlling loading,
2. retaining protein functionality, and
3. creating multi-functional constructs composed of
two or more proteins/particles in a single reaction.
Methods
Mix&Go is an aqueous solution that is directly applied
to synthetic surfaces and incubated at room temp. for
60 min. Surface pre-treatment is not required (e.g.
COC/COP plasma treatment for passive adsorption)
and both particles (Fig 2. A) and planar surfaces (Fig 2.
B) can be activated in a similar manner. Once activated,
surfaces may be stored long term or directly coupled
with protein solution.
A.
B.
Figure 2. Mix&Go activation and protein coupling.
A. Particles may be activated and coupled in as little as 2 hr
compared to 16 hr typically required when using either Amide or
Tosyl chemistries. B. Planar surfaces such as 96-well ELISA
plates may be activated, coupled and blocked in 2.5 hr compared
to overnight coating protocols typical for passive adsorption of
proteins onto ELISA plates.
