"Nanoparticles incorporating different functions are useful and necessary products for assays, drug delivery, and other life science applications. For example, magnetic nanoparticles can be used as contrast agents for magnetic resonance imaging (MRI), to dissipate energy under an oscillating field to locally raise temperature (hyperthermia), or to improve manufacturing of complex nanoparticles via use of magnetic separation. One or more different antibodies and/or fluorescence, luminescence agents as well as other functionalities such as catalytic or enzymatic groups can be attached to nanoparticles.
However, achieving reproducible and controllable addition of just one protein (let alone two or more different proteins) into ever smaller particles is a major challenge - and a technically complicated process. When additional requirements, such as the need to prevent aggregation and to maintain size uniformity are added, simple procedure compatible with many different particles are difficult to find."
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