The pre-print no peer review study was all done in a computer using No humans , or even mice.
Damm they didnt even do it in a petri dish.
Extract
Analysis of the Effectiveness of Promising Drugs: We analyzed ten promising drugs targeting the main (3CL) protease protein of SARS-CoV-2including Nirmatrelvir, Ritonvir, Ivermectin, Lopinavir, Boceprevir, MPro 13b, MPro N3, GC-373, GC376, and PF-00835231 (Jin et al., 2020; Zhang et al., 2020; Vandyck and Deval, 2021).The PDB structures of these drugs were retrieved from the DrugBank (https://go.drugbank.com/)and PubChem (https://pubchem.ncbi.nlm.nih.gov/) (Wishart et al., 2018; Kim et al., 2016). The3D structure of the wild main protease was retrieved from PDB ID: 6WTK. We modeled themutant by SWISS-MODEL (Kiefer et al., 2009). Prior to the molecular docking, we removedwater molecules, ligands, and other complex molecules from the 3D structures. Polar hydrogenatoms and required charges for the energy minimization were further added. Molecular dockingwas performed by using AutoDock Vina tools (Trott and Olson, 2010). We set parameters of thegrid box to size 40 Å × 64 Å × 64 Å (x, y, and z) and center -16.773 × -15.229 × 13.709 (x, y, andz). Further, we used PyMOL for the analysis and visualization of the protein-ligand complexmolecules (DeLano, 2002). The 2D diagrams of Protein-ligand interaction were generated withDiscovery Studio (Biovia, 2017).
