ADO 0.00% 2.1¢ anteotech ltd

This device is a single use device .. it is not currently for...

  1. 3,308 Posts.
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    This device is a single use device .. it is not currently for sale .. boat not leaving.. still a long way to go from proof of concept and why buy a single use test? is will be obselete overnight when a device emerges that can do multiple test with a throw away cartridge and also be linked into the patient/data network (wonder who is doing that).. really swami you are not getting the forward thinking of what TRUE HHPOC devices are.. but at least you are doing some research so hats off to you..

    oh and what for it .. how long does it take to prepare it ??.. thats right buddy 20hours to get one test vial and particles activated and ready.. do you have any idea how quickly Mix&Go can do it ?? clearly not..

    Preparation of the Capture-Antibody-Immobilized Glass Vial
    A glass vial was cleaned by soaking in a mixture of hydrochloric acid and methanol for 1 h and then rinsed several times with methanol and water.(14) After drying under a nitrogen flow, the glass vial was treated with 1% APTES in ethanol for 12 h to produce amine groups on the inner surface of the vial. A 1 mL, 4 μL/mL TnI antibody (4T21 560) solution was added to the glass vial, and the glass vial surface was functionalized with the antibodies using glutaraldehyde as a cross-linker. The glass vial surface was then blocked with a mixture of 1% BSA and 1% Tween 20 at 4 °C for 6 h to prevent nonspecific binding.
    Preparation of Detection-Antibody-Functionalized Platinum Nanoparticles
    Dendritic platinum nanoparticles were synthesized by mixing 20 mL of 1.45 mM H2PtCl6, 250 μL of 1 M ascorbic acid, and 150 μL of 1 M sodium hydroxide.(15) After the solution was heated at 60 °C for 10 min, the solution color changed from pale yellow to brown. The synthesized platinum nanoparticles were purified several times by centrifugation at 14000 rpm for 5 min. A half-fragment detection antibody (4T21 19C7)(16) for easy immobilization of the antibody onto platinum nanoparticles with the desirable orientation was obtained by adding 10 μL of 5 mM TCEP in PB to 400 μL of PB containing 10 μg of detection antibody and incubating for 1 h at room temperature. After immobilization of the half-fragment antibody on platinum nanoparticles via Pt–S bonds, the functionalized nanoparticles were rinsed with PB solution several times, then 0.1% Tween 20 and sucrose were added to the solution to prevent nonspecific binding.

    Mate make sure you read up on these things properly before you post please.. nice try tho..
 
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