published report , page-3

I am glad to see the Author Information:

Ooi HW1, Cooper SJ2, Huang CY2, Jennins D2, Chung E2, Joe Maeji N2, Whittaker AK3.

Author information
1Anteo Diagnostics Limited, Brisbane Technology Park, Unit 4, 26 Brandl St., Eight Mile Plains, 4113 QLD, Australia; Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, Australia.

2Anteo Diagnostics Limited, Brisbane Technology Park, Unit 4, 26 Brandl St., Eight Mile Plains, 4113 QLD, Australia.

3Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, Australia; Centre for Advanced Imaging, University of Queensland, Brisbane, Australia. Electronic address: [email protected].

Abstract
A novel metal-based chelating method has been used to provide an order of magnitude increase in immunoassay performance on COC plastics compared to passive binding. COCs are hydrophobic and without surface modification are often unsuitable for applications where protein adhesion is desired. When interacting with the bare plastic, the majority of the bound proteins will be denatured and become non-functional. Many of the surface modification techniques reported to-date require costly equipment setup or the use of harsh reaction conditions. Here, we have successfully demonstrated the use of a simple and quick metal chelation method to increase the sensitivity, activity and efficiency of protein binding to COC surfaces. A detailed analysis of the COC surfaces after activation with the metal complexes is presented, and the immunoassay performance was studied using three different antibody pairs.
Copyright © 2014. Published by Elsevier Inc.