The latest sequence release shows further Tamiflu resistance in Japan - we can assume that soon all anti virals used in Japan will be Relenza or LANI
pH1N1 Tamiflu Resistant 2010 Sequences In Nagasaki Recombinomics Commentary 13:12 April 1, 2010 The University of Nagasaki has released 13 HA sequences collected in 2010. Although the characterization sheets do not address H274Y in the NA sequences from these isolates, recent releases by NIID has raised concerns that the level of H274Y in recent isolates from Japan is high. Last month 10 of13 NA sequences had H274Y and this month 15 of 15 sequences had H274Y. Since the HA sequences were released from these isolates, phylogenetic analysis of the HA sequences can be used to identify branches with a high level of isolates with H274Y. Of the 13 sequences released by the University of Nagasaki, 8 fell on two branches and both branches had clustering of H274Y isolates (see list below), suggesting that these samples from Nagasaki also had H274Y, either as the dominant species or a significant minor species..
The phylogenetic analysis of sequences from the H274Y positive samples indicates that H274Y is widespread and transmitting in Japan, especially in recent sequences. Of the seven 2010 isolates that have been sequenced for H274Y, six are positive. These latest HA sequences suggest that a high frequency of H274Y also applies to 2010 sequences from Nagasaki, providing further evidence that H274Y is transmitting and becoming fixed in Japan.
Sequences that map with Fukushima/452/2009*, Tochigi/609/2009*, Tochigi/612/2009*
