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clock Created with Sketch. 12/03/24
14:25
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Originally posted by JB1975:
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Just whimsically passing by and these sentences caught me eye ... "MSC isolation and culture from both autologous and allogeneic sources is being hampered by cellular heterogeneity and replicative senescence. Generation of MSC from induced pluripotent stem cells (iPSC) so called MCA -derived MSC may circumvent these limitations" Blue bit - wtf does MCA stand for - m esenchymal c ell A (???). Yellow bit read as true - but really? If autologous (ie cells from the patient themselves) once isolated (so obtained in the pure-est form of the cell type sought - - its isolated its a lone cell - so you have just exactly the cell you want from the patient themselves and nothing else or at least no other cells along for the ride), then cultured (which necessarily involves growing the cell, feeding it with nutrients, keeping it happy so it grows and then divides to form two daughter cells just like their mum) you aren't altering the cell by hitting it with cytokines, its an adherent cell so it adheres to the surface of whatever it is cultured on--- all that
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MCA -maybe mesenchymal cells from autologous sources - so specifically ipscs where the four factors involved in doing the induction of pluripotency are applied to an autologous source and what is then done is that the ipscs are directed towards a mesenchymal fate --- is that what is going on there ?
(20min delay)