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manuva13

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15/11/16

23:42

Post #: 20642843
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Originally posted by nihilism ↑

I see your point, but I disagree when you say MALDI-TOF was 100% for all... because while it was very specific, it had the opposite problem, being sensitivity too low.

View attachment 337952

Some of which were around 60%, which is far lower than any individual APAS test.

I think holding any test up as the holy grail is foolish, that's not how the industry works. I think there is a huge usefulness in the APAS, and your information provided confirmed my thoughts.

The point I was making around APAS is depending on the parameters in the algorithm you should be able to segregate results into (for instance)

VP - extremely positive, definite growth of definitive type.
MP - mild positive - definite growth of unknown type or unknown growth of definite type
FR - for review - non-definite growth of indefinite type
VN - very negative.

APAS algorithms can then be tuned such that VP and VN can be removed. This will take some trial and error and will likely have review stages for a few years, but if you cannot see the commercial benefit to the laboratories of automating this process and removing 40-60% of plates from review (or being able to process double the plates) then I think you're too involved in the technical flaws, rather than the business opportunity.

I look at it as a business, the technology isn't perfect, nor will it replace technicians in the near future, but it can increase technician productivity.

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I totally see the commercial benefit, it will be an excellent machine to screen large numbers of plates and remove that 70% of negative plates I waste my time with!.. But it won't be used to report confirmed, finalised bacterial IDs. Having gone through the LBT patents today I'm even more confident in that assertion.

MALDI-TOF in the micro lab is not used in a sensitive capacity, it is used exclusively for it's specificity, for it's certainty in bacterial ID...The technician/scientist provides the sensitivity, identifying all positive plates for work-up.

Digital imaging/screening + MALDI-TOF = the future of automationn

LBT Price at posting: 49.0¢ Sentiment: Buy Disclosure: Held
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